Parallelized Immunomagnetic Isolation of Basophils Directly from Whole Blood.

ABSTRACT: Basophils are the rarest circulating white blood cells (WBCs), but they play important roles in allergic disorders and other diseases. To enhance diagnostic capabilities, it would be desirable to isolate and analyze basophils efficiently from small blood samples. In 100 μL of whole blood, there are typically ~10³ basophils, outnumbered by ~10⁵ WBCs and ~10⁸ red blood cells (RBCs). Basophils' low abundance has therefore presented a significant challenge in their isolation from whole blood. Conventional in-bulk basophil isolation methods require lengthy processing steps and cannot work with small volumes of blood. Here we report a parallelized integrated basophil isolation device (pi-BID) for the negative immunomagnetic selection of basophils directly from 4

SUBMITTER: Myles J 

PROVIDER: S-EPMC11244651 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature