Spontaneous single-nucleotide substitutions and microsatellite mutations have distinct distributions of fitness effects.

ABSTRACT: The fitness effects of new mutations determine key properties of evolutionary processes. Beneficial mutations drive evolution, yet selection is also shaped by the frequency of small-effect deleterious mutations, whose combined effect can burden otherwise adaptive lineages and alter evolutionary trajectories and outcomes in clonally evolving organisms such as viruses, microbes, and tumors. The small effect sizes of these important mutations have made accurate measurements of their rates difficult. In microbes, assessing the effect of mutations on growth can be especially instructive, as this complex phenotype is closely linked to fitness in clonally evolving organisms. Here, we perform high-throughput time-lapse microscopy on cells from mutation-accumulation strains to precisely infer the d

SUBMITTER: Plavskin Y 

PROVIDER: S-EPMC11244821 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature