Measurement of covalent bond formation in light-curing hydrogels predicts physical stability under flow.

ABSTRACT: Photocrosslinking hydrogels are promising for tissue engineering and regenerative medicine, but challenges in reaction monitoring often leave their optimization subject to trial and error. The stability of crosslinked gels under fluid flow, as in the case of a microfluidic device, is particularly challenging to predict, both because of obstacles inherent to solid-state macromolecular analysis that prevent accurate chemical monitoring, and because stability is dependent on size of the patterned features. To solve both problems, we obtained ¹H NMR spectra of cured hydrogels which were enzymatically degraded. This allowed us to take advantage of the high-resolution that solution NMR provides. This unique approach enabled the measurement of degree of crosslinking (DoC) and predictio

SUBMITTER: Zatorski JM 

PROVIDER: S-EPMC11244878 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature