Spatial transcriptomics reveals influence of microenvironment on intrinsic fates in melanoma therapy resistance.

ABSTRACT: Resistance to cancer therapy is driven by both cell-intrinsic and microenvironmental factors. Previous work has revealed that multiple resistant cell fates emerge in melanoma following treatment with targeted therapy and that, in vitro, these resistant fates are determined by the transcriptional state of individual cells prior to exposure to treatment. What remains unclear is whether these resistant fates are shared across different genetic backgrounds and how, if at all, these resistant fates interact with the tumor microenvironment. Through spatial transcriptomics and single-cell RNA sequencing, we uncovered distinct resistance programs in melanoma cells shaped by both intrinsic cellular states and the tumor microenvironment. Consensus non-negative matrix factorization revealed sh

SUBMITTER: Boe RH 

PROVIDER: S-EPMC11244927 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature