Developing, characterizing and modeling CRISPR-based point-of-use pathogen diagnostics.

ABSTRACT: Recent years have seen intense interest in the development of point-of-care nucleic acid diagnostic technologies to address the scaling limitations of laboratory-based approaches. Chief among these are combinations of isothermal amplification approaches with CRISPR-based detection and readouts of target products. Here, we contribute to the growing body of rapid, programmable point-of-care pathogen tests by developing and optimizing a one-pot NASBA-Cas13a nucleic acid detection assay. This test uses the isothermal amplification technique NASBA to amplify target viral nucleic acids, followed by Cas13a-based detection of amplified sequences. We first demonstrate an in-house formulation of NASBA that enables optimization of individual NASBA components. We then present design rules for NASBA pr

SUBMITTER: Jung JK 

PROVIDER: S-EPMC11244977 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature