High Throughput Repurposing Screen Reveals Compounds with Activity Against <i>Toxoplasma gondii</i> Bradyzoites.

ABSTRACT: Toxoplasma gondii causes widespread chronic infections that are not cured by current treatments due to inability to affect semi-dormant bradyzoite stages within tissue cysts. To identify compounds to eliminate chronic infection, we developed a HTS using a recently characterized strain of T. gondii that undergoes efficient conversion to bradyzoites in intro. Stage-specific expression of luciferase was used to selectively monitor growth inhibition of bradyzoites by the Library of Pharmacological Active Compounds, consisting of 1,280 drug-like compounds. We identified 44 compounds with >50% inhibitory effects against bradyzoites, including new highly potent compounds, several of which have precedent for antimicrobial activity. Subsequent characterization of the compound Sanguina

SUBMITTER: Uddin T 

PROVIDER: S-EPMC11244992 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature