Accounting for differences between Infinium MethylationEPIC v2 and v1 in DNA methylation-based tools.

ABSTRACT: The recently launched Illumina Infinium MethylationEPIC v2.0 (EPICv2), successor of MethylationEPIC v1.0 (EPICv1), retains a majority of probes in EPICv1, while expanding coverage of regulatory elements. The concordance between the two EPIC versions in DNA methylation-based tools has not yet been investigated. To address this, DNA methylation was profiled on both versions using matched blood samples across four cohorts spanning early to late adulthood. High concordance between versions at the array level but variable agreement at the individual probe level was noted. A significant contribution of EPIC version to DNA methylation variation was observed, though it was to a smaller extent compared to sample relatedness and cell type composition. Modest but significant differences in DNA methyl

SUBMITTER: Zhuang BC 

PROVIDER: S-EPMC11245009 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature