KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription.

ABSTRACT: Signal-induced transcriptional programs regulate critical biological processes through the precise spatiotemporal activation of Immediate Early Genes (IEGs); however, the mechanisms of transcription induction remain poorly understood. By combining an acute depletion system with several genomics approaches to interrogate synchronized, temporal transcription, we reveal that KAP1/TRIM28 is a first responder that fulfills the temporal and heightened transcriptional demand of IEGs. Acute KAP1 loss triggers an increase in RNA polymerase II elongation kinetics during early stimulation time points. This elongation defect derails the normal progression through the transcriptional cycle during late stimulation time points, ultimately leading to decreased recruitment of the transcription apparatus fo

SUBMITTER: Hyder U 

PROVIDER: S-EPMC11245487 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature