Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing.

ABSTRACT: Bead array assays, such as those sold by Luminex, BD Biosciences, Sartorius, Abcam and other companies, are a well-established platform for multiplexed quantification of cytokines and other biomarkers in both clinical and discovery research environments. In 2011, the National Institute of Allergy and Infectious Diseases (NIAID)-funded External Quality Assurance Program Oversight Laboratory (EQAPOL) established a proficiency assessment program to monitor participating laboratories performing multiplex cytokine measurements using Luminex bead array technology. During every assessment cycle, each site was sent an assay kit, a protocol, and blinded samples of human sera spiked with recombinant cytokines. Site results were then evaluated for performance relative to peer laboratories. After over

SUBMITTER: Rountree W 

PROVIDER: S-EPMC11246216 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature