A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings.

ABSTRACT: Stable genetic transformation of peach [Prunus persica (L.) Batsch] still faces many technical challenges, and existing transient expression methods are limited by tissue type or developmental stage, making it difficult to conduct functional analysis of genes regulating shoot growth. To overcome this dilemma, we developed a three-step method for efficient analysis of gene functions during peach seedling growth and development. This method resulted in transformation frequencies ranging from 48 to 87%, depending on the gene. From transformation of germinating seeds to phenotyping of young saplings took just 1.5 months and can be carried out any time of year. To test the applicability of this method, the function of three tree architecture-related genes, namely PpPDS, PpMAX4<

SUBMITTER: Cheng J 

PROVIDER: S-EPMC11246241 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature