An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis.

ABSTRACT: The early phases of clathrin mediated endocytosis are organized through a highly complex interaction network mediated by clathrin associated sorting proteins (CLASPs) that comprise long intrinsically disordered regions (IDRs). AP180 is a CLASP exclusively expressed in neurons and comprises a long IDR of around 600 residues, whose function remains partially elusive. Using NMR spectroscopy, we discovered an extended and strong interaction site within AP180 with the major adaptor protein AP2, and describe its binding dynamics at atomic resolution. We find that the 70 residue-long site determines the overall interaction between AP180 and AP2 in a dynamic equilibrium between its bound and unbound states, while weaker binding sites contribute to the overall affinity at much higher concentrations

SUBMITTER: Naudi-Fabra S 

PROVIDER: S-EPMC11246429 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature