M¹A demethylase Alkbh3 regulates neurogenesis through m¹A demethylation of Mmp15 mRNA.

ABSTRACT:

Background

N¹-Methyladenosine (m¹A) is an abundant modification of transcripts regulating mRNA structure and translation efficiency. However, the characteristics and biological functions of mRNA m¹A modification in adult hippocampal neurogenesis remain enigmatic.

Results

We found that m¹A demethylase Alkbh3 was dramatically enriched in neurons and neuronal genesis. Functionally, depletion of Alkbh3 in neural stem cells (NSCs) significantly decreased m¹A modification, neuronal differentiation and proliferation coupling with increasing gliogenesis, whereas overexpressing Alkbh3 facilitated neuronal differentiation and proliferation. Mechanistically, the m¹A demethylation of Mmp15 mRNA by Alkbh3 improved its RNA

SUBMITTER: Wang H 

PROVIDER: S-EPMC11246583 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature