Gut microbiome-immune interactions and their role in rheumatoid arthritis development.

ABSTRACT:

Objective

The primary objective is to study the impact of gut microbiota and their interactions with diverse immunological markers on the development of rheumatoid arthritis.

Methods

This study was performed in Astana, Kazakhstan, and included 77 Kazakh female patients older than 18 years, who met the American College of Rheumatology 2010 classification criteria for rheumatoid arthritis (RA), and 113 healthy controls. The DNA was extracted from fecal samples obtained from all study participants for subsequent sequencing at the 16S rRNA gene V1-V3 locus, facilitating the analysis of the gut microbiome. The Multiplex immunoassay was employed to measure the concentrations of inflammatory cytokines, chemokines, and immunoglobulins in both fecal and plasma samples.

Results

SUBMITTER: Nurgaziyev M 

PROVIDER: S-EPMC11246623 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature