Achieving efficient clonal beta cells transfection using nanostraw/nanopore-assisted electroporation.

ABSTRACT: The prospect of being able to efficiently inject large plasmids in insulin-producing beta cells is very attractive for diabetes research. However, conventional transfection methods suffer from high cytotoxicity or low transfection efficiency, which negatively affect their outcome. In contrast, nanostraw electroporation is a gentle method that can provide a high transfection efficiency while maintaining high cell viability. While nanostraw electroporation has gone through some method optimization in the past, such as tuning the pulse frequency, amplitude, and duration, the effect of other parameters has not been thoroughly investigated. Here, we demonstrate efficient transfection of clonal beta cells and investigate the effect of voltage at a fixed inter-electrode distance, cell density, an

SUBMITTER: Ekstrand F 

PROVIDER: S-EPMC11247384 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature