Quantifying in vivo collagen reorganization during immunotherapy in murine melanoma with second harmonic generation imaging.

ABSTRACT:

Significance

Increased collagen linearization and deposition during tumorigenesis can impede immune cell infiltration and lead to tumor metastasis. Although melanoma is well studied in immunotherapy research, studies that quantify collagen changes during melanoma progression and treatment are lacking.

Aim

We aim to image in vivo collagen in preclinical melanoma models during immunotherapy and quantify the collagen phenotype in treated and control mice.

Approach

Second-harmonic generation imaging of collagen was performed in mouse melanoma tumors in vivo over a treatment time course. Animals were treated with a curative radiation and immunotherapy combination. Collagen morphology was quantified over time at an image and single-fiber level using CurveAlign

SUBMITTER: Heaton AR 

PROVIDER: S-EPMC11247620 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature