Characterization of neurofibrillary tangle immunophenotype signatures to classify tangle maturity in Alzheimer's disease.

ABSTRACT:

Introduction

Tau aggregation into neurofibrillary tangles in Alzheimer's disease (AD) is a dynamic process involving changes in tau phosphorylation, isoform composition, and morphology. To facilitate studies of tangle maturity, we developed an image analysis pipeline to study antibody labeling signatures that can distinguish tangle maturity levels in AD brain tissue.

Methods

Using fluorescent immunohistochemistry, we co-labeled AD brain tissue with four antibodies that bind different tau epitopes. Mean fluorescence intensity of each antibody was measured, and spectral clustering was used to identify tangle immunophenotypes.

Results

Five distinct tangle populations were identified, and different tangle maturity immunophenotypes were identified with increasing Braak sta

SUBMITTER: Hamlin D 

PROVIDER: S-EPMC11247694 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature