TOTEM: a multi-cancer detection and localization approach using circulating tumor DNA methylation markers.

ABSTRACT:

Background

Detection of cancer and identification of tumor origin at an early stage improve the survival and prognosis of patients. Herein, we proposed a plasma cfDNA-based approach called TOTEM to detect and trace the cancer signal origin (CSO) through methylation markers.

Methods

We performed enzymatic conversion-based targeted methylation sequencing on plasma cfDNA samples collected from a clinical cohort of 500 healthy controls and 733 cancer patients with seven types of cancer (breast, colorectum, esophagus, stomach, liver, lung, and pancreas) and randomly divided these samples into a training cohort and a testing cohort. An independent validation cohort of 143 healthy controls, 79 liver cancer patients and 100 stomach cancer patients were recruited to validate the gene

SUBMITTER: Xiong D 

PROVIDER: S-EPMC11247868 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature