Diaph1 knockout inhibits mouse primordial germ cell proliferation and affects gonadal development.

ABSTRACT:

Background

Exploring the molecular mechanisms of primordial germ cell (PGC) migration and the involvement of gonadal somatic cells in gonad development is valuable for comprehending the origins and potential treatments of reproductive-related diseases.

Methods

Diaphanous related formin 1 (Diaph1, also known as mDia1) was screened by analyzing publicly available datasets (ATAC-seq, DNase-seq, and RNA-seq). Subsequently, the CRISPR-Cas9 technology was used to construct Diaph1 knockout mice to investigate the role of Diaph1 in gonad development.

Results

Based on data from public databases, a differentially expressed gene Diaph1, was identified in the migration of mouse PGC. Additionally, the number of PGCs was significantly reduced in Diaph1 knockout mice compared to wil

SUBMITTER: Zhao X 

PROVIDER: S-EPMC11247884 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature