Milk fat globule-epidermal growth factor 8 (MFGE8) prevents intestinal fibrosis.

ABSTRACT:

Objective

Intestinal fibrosis is considered an inevitable consequence of chronic IBD, leading to stricture formation and need for surgery. During the process of fibrogenesis, extracellular matrix (ECM) components critically regulate the function of mesenchymal cells. We characterised the composition and function of ECM in fibrostenosing Crohn's disease (CD) and control tissues.

Design

Decellularised full-thickness intestinal tissue platforms were tested using three different protocols, and ECM composition in different tissue phenotypes was explored by proteomics and validated by quantitative PCR (qPCR) and immunohistochemistry. Primary human intestinal myofibroblasts (HIMFs) treated with milk fat globule-epidermal growth factor 8 (MFGE8) were evaluated regarding the mechanis

SUBMITTER: Lin S 

PROVIDER: S-EPMC11248270 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature