Conformational transitions in redissolved silk fibroin films and application for printable self-powered multistate resistive memory biomaterials.

ABSTRACT: 3D printing of water stable proteins with elastic properties offers a broad range of applications including self-powered biomedical devices driven by piezoelectric biomaterials. Here, we present a study on water-soluble silk fibroin (SF) films. These films were prepared by mixing degummed silk fibers and calcium chloride (CaCl₂) in formic acid, resulting in a silk I-like conformation, which was then converted into silk II by redissolving in phosphate buffer (PBS). Circular dichroism, Raman and infrared (IR) spectroscopies were used to investigate the transitions of secondary structure in silk I and silk II as the pH of the solvent and the sonication time were changed. We showed that a solvent with low pH (e.g. 4) maintains the silk I β-turn structure; in contrast solvent

SUBMITTER: Libera V 

PROVIDER: S-EPMC11248567 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature