Multimodal analysis of traction forces and the temperature dynamics of living cells with a diamond-embedded substrate.

ABSTRACT: Cells and tissues are constantly exposed to chemical and physical signals that regulate physiological and pathological processes. This study explores the integration of two biophysical methods: traction force microscopy (TFM) and optically detected magnetic resonance (ODMR) to concurrently assess cellular traction forces and the local relative temperature. We present a novel elastic substrate with embedded nitrogen-vacancy microdiamonds that facilitate ODMR-TFM measurements. Optimization efforts focused on minimizing sample illumination and experiment duration to mitigate biological perturbations. Our hybrid ODMR-TFM technique yields TFM maps and achieves approximately 1 K precision in relative temperature measurements. Our setup employs a simple wide-field fluorescence microscope with sta

SUBMITTER: Kolodziej T 

PROVIDER: S-EPMC11249686 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature