Dataset Information

Combined DNA Methylation and Gastric Microbiome Marker Predicts Helicobacter pylori-Negative Gastric Cancer.

ABSTRACT:

Background/aims

While DNA methylation and gastric microbiome are each associated with gastric cancer (GC), their combined role in predicting GC remains unclear. This study investigated the potential of a combined DNA methylation and gastric microbiome signature to predict Helicobacter pylori-negative GC.

Methods

In this case-control study, we conducted quantitative methylation-specific polymerase chain reaction to measure the methylation levels of DKK3, SFRP1, EMX1, NKX6-1, MIR124-3, and TWIST1 in the gastric mucosa from 75 H. pylori-negative patients, including chronic gastritis (CG), intestinal metaplasia (IM), and GC. A combined analysis of DNA methylation and gastric microbiome, using 16S rRNA gene sequencing, was performed in 30 of 75 patients.

Results

The methylation levels of DKK3, SFRP1, EMX1, MIR124-3, and TWIST1 were significantly higher in patients with GC than in controls (all q<0.05). MIR124-3 and TWIST1 methylation levels were higher in patients with IM than those with CG and also in those with GC than in those with IM (all q<0.05). A higher methylation level of TWIST1 was an independent predictor for H. pylori-negative GC after adjusting for age, sex, and atrophy (odds ratio [OR], 15.15; 95% confidence interval [CI], 1.58 to 145.46; p=0.018). The combination of TWIST1 methylation and GC microbiome index (a microbiome marker) was significantly associated with H. pylori-negative GC after adjusting for age, sex, and atrophy (OR, 50.00; 95% CI, 1.69 to 1,476; p=0.024).

Conclusions

The combination of TWIST1 methylation and GC microbiome index may offer potential as a biomarker for predicting H. pylori-negative GC.

SUBMITTER: Kim MJ

PROVIDER: S-EPMC11249944 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Publications

Combined DNA Methylation and Gastric Microbiome Marker Predicts <i>Helicobacter pylori</i>-Negative Gastric Cancer.

Kim Min-Jeong MJ Kim Han-Na HN Jacobs Jonathan P JP Yang Hyo-Joon HJ

Gut and liver 20240321 4

<h4>Background/aims</h4>While DNA methylation and gastric microbiome are each associated with gastric cancer (GC), their combined role in predicting GC remains unclear. This study investigated the potential of a combined DNA methylation and gastric microbiome signature to predict <i>Helicobacter pylori</i>-negative GC.<h4>Methods</h4>In this case-control study, we conducted quantitative methylation-specific polymerase chain reaction to measure the methylation levels of <i>DKK3</i>, <i>SFRP1</i>, ...[more]

PMID: 38509701

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