Azole resistance screening in <i>Aspergillus fumigatus sensu stricto</i> using the azole-containing agar method (EUCAST E.Def 10.2): conidial suspension filtration and inoculum adjustment before inoculum preparation may not be needed.

ABSTRACT: Azole resistance screening in Aspergillus fumigatus sensu stricto can be routinely carried out by using azole-containing agar plates (E.Def 10.2 procedure); however, conidial suspension filtering and inoculum adjustment before inoculum preparation are time-consuming. We evaluated whether skipping the filtration and inoculum adjustment steps negatively influenced the performance of the E.Def 10.2 procedure. A. fumigatus sensu stricto isolates (n = 98), previously classified as azole susceptible or azole resistant (E.Def 9.4 method), were studied. Azole-resistant isolates had either the wild-type cyp51A gene sequence (n = 1) or the following cyp51A gene substitutions: TR₃₄-L98H (n = 41), G54R (n = 5), TR₄₆-Y121F-T2

SUBMITTER: Serrano-Lobo J 

PROVIDER: S-EPMC11250424 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature