A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery.

ABSTRACT: Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.

SUBMITTER: Jeremiasse B 

PROVIDER: S-EPMC11251264 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature