RNA-seq validation: software for selection of reference and variable candidate genes for RT-qPCR.

ABSTRACT:

Background

Real-time quantitative PCR (RT-qPCR) is one of the most widely used gene expression analyses for validating RNA-seq data. This technique requires reference genes that are stable and highly expressed, at least across the different biological conditions present in the transcriptome. Reference and variable candidate gene selection is often neglected, leading to misinterpretation of the results.

Results

We developed a software named "Gene Selector for Validation" (GSV), which identifies the best reference and variable candidate genes for validation within a quantitative transcriptome. This tool also filters the candidate genes concerning the RT-qPCR assay detection limit. GSV was compared with other software using synthetic datasets and performed better, removing stab

SUBMITTER: de Brito MWD 

PROVIDER: S-EPMC11251314 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature