PRRX1-OLR1 axis supports CAFs-mediated lung cancer progression and immune suppression.

ABSTRACT:

Objective

To investigate the mechanism by which cancer-associated fibroblasts (CAFs) affect the growth and immune evasion of lung cancer cells.

Methods

Initially, datasets comparing CAFs with normal fibroblasts were downloaded from the GEO dataset GSE48397. Genes with the most significant differential expression were selected and validated using clinical data. Subsequently, CAFs were isolated, and the selected genes were knocked down in CAFs. Co-culture experiments were conducted with H1299 or A549 cells to analyze changes in lung cancer cell growth, migration, and immune evasion in vitro and in vivo. To further elucidate the upstream regulatory mechanism, relevant ChIP-seq data were downloaded from the GEO database, and the regulatory relationships were validated through Ch

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11251326 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature