Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii.

ABSTRACT:

Background

Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single-carbon feedstocks, derived from CO₂, represent promising substrates for biotechnological applications. Especially, methanol is gaining prominence for bio-production of commodity chemicals.

Results

In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus (cadA, mttA and mfsA) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g·L^-1 after 5 days of fed-

SUBMITTER: Severinsen MM 

PROVIDER: S-EPMC11251334 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature