Context-dependent regulation of lipid accumulation in adipocytes by a HIF1α-PPARγ feedback network.

ABSTRACT: Hypoxia-induced upregulation of HIF1α triggers adipose tissue dysfunction and insulin resistance in obese patients. HIF1α closely interacts with PPARγ, the master regulator of adipocyte differentiation and lipid accumulation, but there are conflicting results regarding how this interaction controls the excessive lipid accumulation that drives adipocyte dysfunction. To directly address these conflicts, we established a differentiation system that recapitulated prior seemingly opposing observations made across different experimental settings. Using single-cell imaging and coarse-grained mathematical modeling, we show how HIF1α can both promote and repress lipid accumulation during adipogenesis. Our model predicted and our experiments confirmed that the opposing roles of HIF1α are isolated fr

SUBMITTER: Kudo T 

PROVIDER: S-EPMC11251692 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature