Comparison three primer pairs for molecular sex determination in Eurasian pygmy owls (Glaucidium passerinum).

ABSTRACT: Bird sex determination is fundamental in various ecological and biological studies, although many avian species cannot be sexed visually due to their monomorphic and/or monochromatic appearance. Thus, reliable laboratory methods for sexing are a prerequisite. Most avian nestlings lack sex-related signs, including the Eurasian pygmy owl (Glaucidium passerinum). We performed laboratory sex determination analysis of this species using blood samples of 242 juveniles and nine adults. It relied on the qPCR of the specific intron from the chromo-helicase DNA-binding protein 1 gene. We tested three primer sets, the P2/P8, 2550F/2718R, and CHD1F/CHD1R, commonly used for bird laboratory sexing. The outcomes were displayed on an agarose gel electrophoresis and a plot from melt curve analysis, which h

SUBMITTER: Stehlikova Sovadinova S 

PROVIDER: S-EPMC11252122 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature