Genetic features of BEL-1-producing and KPC-2-producing E. coli from hospital wastewater: human source or sewages adaptation.

ABSTRACT: Hospital sewage is an ecosystem that facilitates the transfer of antibiotic and heavy metal resistance genes and the interaction of human and environmental bacteria. In this environment, we have detected the presence of 7 KPC-2 and BEL-1 co-producing E. coli isolates of two different clones over a 10-month period in the same hospital. All isolates carried bla_KPC-2 and the operon mer on the same IncP plasmid of similar size and an IncN plasmid of different size each clone carrying bla_BEL-1. Both IncN-blaBEL-1 plasmids shared a 77 kb region containing bla_BEL-1 alongside with fosE, bla _OXA-10 and aac(6')-1b genes in a class 3 integron within a Tn3 transposon. The major IncN plasmid contained in addition a region homolog to P1-like bacteriophage RCS4

SUBMITTER: Romero-Oraa L 

PROVIDER: S-EPMC11252172 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature