Introducing carbon assimilation in yeasts using photosynthetic directed endosymbiosis.

ABSTRACT: Conversion of heterotrophic organisms into partially or completely autotrophic organisms is primarily accomplished by extensive metabolic engineering and laboratory evolution efforts that channel CO₂ into central carbon metabolism. Here, we develop a directed endosymbiosis approach to introduce carbon assimilation in budding yeasts. Particularly, we engineer carbon assimilating and sugar-secreting photosynthetic cyanobacterial endosymbionts within the yeast cells, which results in the generation of yeast/cyanobacteria chimeras that propagate under photosynthetic conditions in the presence of CO₂ and in the absence of feedstock carbon sources like glucose or glycerol. We demonstrate that the yeast/cyanobacteria chimera can be engineered to biosynthesize natural product

SUBMITTER: Gao YL 

PROVIDER: S-EPMC11252298 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature