Revealing the atomic and electronic mechanism of human manganese superoxide dismutase product inhibition.

ABSTRACT: Human manganese superoxide dismutase (MnSOD) is a crucial oxidoreductase that maintains the vitality of mitochondria by converting superoxide (O₂^●-) to molecular oxygen (O₂) and hydrogen peroxide (H₂O₂) with proton-coupled electron transfers (PCETs). Human MnSOD has evolved to be highly product inhibited to limit the formation of H₂O₂, a freely diffusible oxidant and signaling molecule. The product-inhibited complex is thought to be composed of a peroxide (O₂^2-) or hydroperoxide (HO₂^-) species bound to Mn ion and formed from an unknown PCET mechanism. PCET mechanisms of proteins are typically not known due to difficulties in detecting the protonation states of specific r

SUBMITTER: Azadmanesh J 

PROVIDER: S-EPMC11252399 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature