Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments.

ABSTRACT: Maintenance of water homeostasis is a fundamental cellular process required by all living organisms. Here, we use the single-celled green alga Chlamydomonas reinhardtii to establish a foundational understanding of osmotic-stress signaling pathways through transcriptomics, phosphoproteomics, and functional genomics approaches. Comparison of pathways identified through these analyses with yeast and Arabidopsis allows us to infer their evolutionary conservation and divergence across these lineages. 76 genes, acting across diverse cellular compartments, were found to be important for osmotic-stress tolerance in Chlamydomonas through their functions in cytoskeletal organization, potassium transport, vesicle trafficking, mitogen-activated protein kinase and chloroplast signaling. We show that ho

SUBMITTER: Vilarrasa-Blasi J 

PROVIDER: S-EPMC11252407 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature