PUF3 RNA binding protein of Trypanosoma cruzi regulates mitochondrial morphology and function.

ABSTRACT: The RNA-binding PUF proteins are post-transcriptional regulators found throughout the eukaryotic domain. In Trypanosoma cruzi, ten Puf genes termed Puf1 to Puf10 have been identified. Considering that the control of gene expression in this parasite is mainly at the post-transcriptional level, we characterized the PUF3 protein by knocking out and overexpressing the gene in T. cruzi epimastigotes and studied different genetic and biological features. The RNA-seq analyses in both genotypes showed significant changes in the number of regulated transcripts compared with wild-type parasites. Thus, the number of differentially expressed genes in the knockout (ΔTcPuf3) and the overexpressor (pTEXTcPuf3) were 238 and 187, respectively. In the knockou

SUBMITTER: Mejia-Jaramillo AM 

PROVIDER: S-EPMC11252720 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature