Epigenetic control of SOX9 gene by the histone acetyltransferase P300 in human Sertoli cells.

ABSTRACT:

Background

The transcription factor SOX9 is a key regulator of male sexual development and Sertoli cell differentiation. Altered SOX9 expression has been implicated in the pathogenesis of disorders of sexual development (DSD) in mammals. However, limited information exists regarding the epigenetic mechanisms governing its transcriptional control during sexual development.

Methods

This study employed real-time PCR (qPCR), immunofluorescence (IIF), and chromatin immunoprecipitation (ChIP) assays to investigate the epigenetic mechanisms associated with SOX9 gene transcriptional control in human and mouse Sertoli cell lines. To identify the specific epigenetic enzymes involved in SOX9 epigenetic control, functional assays using siRNAs for P300, GCN5, and WDR5 were

SUBMITTER: Gonzalez D 

PROVIDER: S-EPMC11252772 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature