A tandem activity-based sensing and labeling strategy reveals antioxidant response element regulation of labile iron pools.

ABSTRACT: Iron is an essential element for life owing to its ability to participate in a diverse array of oxidation-reduction reactions. However, misregulation of iron-dependent redox cycling can also produce oxidative stress, contributing to cell growth, proliferation, and death pathways underlying aging, cancer, neurodegeneration, and metabolic diseases. Fluorescent probes that selectively monitor loosely bound Fe(II) ions, termed the labile iron pool, are potentially powerful tools for studies of this metal nutrient; however, the dynamic spatiotemporal nature and potent fluorescence quenching capacity of these bioavailable metal stores pose challenges for their detection. Here, we report a tandem activity-based sensing and labeling strategy that enables imaging of labile iron pools in live cells

SUBMITTER: Pezacki AT 

PROVIDER: S-EPMC11252945 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature