ScMicrobe PTA: near complete genomes from single bacterial cells.

ABSTRACT: Microbial genomes produced by standard single-cell amplification methods are largely incomplete. Here, we show that primary template-directed amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard multiple displacement amplification-based approaches were usually <30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters.

SUBMITTER: Bowers RM 

PROVIDER: S-EPMC11253033 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature