Suppression of inositol pyrophosphate toxicosis and hyper-repression of the fission yeast PHO regulon by loss-of-function mutations in chromatin remodelers Snf22 and Sol1.

ABSTRACT: Inositol pyrophosphates are signaling molecules that regulate cellular phosphate homeostasis in eukaryal taxa. In fission yeast, where the phosphate regulon (comprising phosphate acquisition genes pho1, pho84, and tgp1) is repressed under phosphate-replete conditions by lncRNA-mediated transcriptional interference, mutations of inositol pyrophosphatases that increase IP₈ levels derepress the PHO regulon by eliciting precocious termination of lncRNA transcription. Asp1 pyrophosphatase mutations resulting in too much IP₈ are cytotoxic in YES medium owing to overexpression of glycerophosphodiester transporter Tgp1. IP₈ toxicosis is ameliorated by mutations in cleavage/polyadenylation and termination factors, perturbations of the Pol

SUBMITTER: Schwer B 

PROVIDER: S-EPMC11253589 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature