Stability and gene strand bias of lambda prophages and chromosome organization in Escherichia coli.

ABSTRACT: Temperate phage-mediated horizontal gene transfer is a potent driver of genetic diversity in the evolution of bacteria. Most lambdoid prophages in Escherichia coli are integrated into the chromosome with the same orientation with respect to the direction of chromosomal replication, and their location on the chromosome is far from homogeneous. To better understand these features, we studied the interplay between lysogenic and lytic states of phage lambda in both native and inverted integration orientations at the wild-type integration site as well as at other sites on the bacterial chromosome. Measurements of free phage released by spontaneous induction showed that the stability of lysogenic states is affected by location and orientation along the chromosome, with stronger effects ne

SUBMITTER: Li X 

PROVIDER: S-EPMC11253608 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature