Spatial recovery of the murine gut microbiota after antibiotics perturbation.

ABSTRACT: Bacterial communities are highly complex, with interaction networks dictating ecosystem function. Bacterial interactions are constrained by the spatial organization of these microbial communities, yet studying the spatial organization of microbial communities at the single-cell level has been technically challenging. Here, we use the recently developed high-phylogenetic-resolution microbiota mapping by fluorescence in situ hybridization technology to image the gut microbiota at the species and single-cell level. We simultaneously image 63 different bacterial species to spatially characterize the perturbation and recovery of the gut microbiota to ampicillin and vancomycin in the cecum and distal colon of mice. To decipher the biology in this complex imaging data, we developed an anal

SUBMITTER: Taguer M 

PROVIDER: S-EPMC11253616 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature