Strain-level variations of Dirofilaria immitis microfilariae in two biochemical assays.

ABSTRACT:

Background

The increase in reports of resistance to macrocyclic lactones in the canine heartworm, Dirofilaria immitis is alarming. While DNA based tests have been well-validated, they can be expensive. In a previous study, we showed that two biochemical tests adapted to a 96- well plate format and read in a spectrophotometer could detect differences among lab validated D. immitis isolates. The two tests- Resazurin reduction and Hoechst 33342 efflux-detect metabolism and P-glycoprotein activity respectively in microfilariae isolated from infected dog blood.

Methods

Our objective was to optimize the two assays further by testing various assay parameters in D. immitis isolates not tested previously. We tested microfilarial seeding density, incubation time and the effect of in v

SUBMITTER: Hampton N 

PROVIDER: S-EPMC11253964 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature