L-valine derived from the gut microbiota protects sepsis-induced intestinal injury and negatively correlates with the severity of sepsis.

ABSTRACT:

Background

The protective role of gut microbiota and its metabolites against intestinal damage in sepsis patients remain unclear.

Methods

Fecal samples were acquired from patients categorized into sepsis and non-sepsis groups for analysis of microbial composition via 16S rRNA sequencing and untargeted metabolomics analysis. We assessed the impact of gut microbiota from sepsis patients on intestinal barriers in antibiotic-treated mice. Furthermore, We conducted spearman's correlation analysis to examine the relationship between metabolites and the severity of sepsis. Additionally, we performed animal experiments to validate the functionality of identified metabolites.

Results

The diversity of intestinal flora is decreased in patients with sepsis compared to the control

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11254637 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature