Soluble CD72, is a T-cell activator probably via binding to CD6 in homeostasis and autoimmunity.

ABSTRACT:

Background

CD72 is a highly required regulatory molecule in B cells. Its sufficient expression is crucial for maintaining self-tolerance. In contrast, soluble CD72 (sCD72) is reported to be increased in the serum of autoimmune diseases such as systemic lupus erythematosus and primary Sjogren's syndrome (pSS).

Objective

We wanted to assess the biological effect of sCD72 on CD4⁺T cells.

Methods

We performed mass spectrometry and co-immunoprecipitation experiments to look for a sCD72 receptor on activated CD4⁺T cells. Afterward, to explore the biological functions of sCD72, we used flow cytometry for the cytokine secretion profile, a phosphorylation assay for the signaling pathway, and a CFSE dye-based assay for cell proliferation.

Results

We

SUBMITTER: Eiza N 

PROVIDER: S-EPMC11254670 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature