Chloroplast genome-based genetic resources via genome skimming for the subalpine forests of Japan and adjacent regions.

ABSTRACT: The Japanese subalpine zone is dominated by an ecologically important forest biome, subalpine coniferous forest, constituting a distinct assemblage of cold-tolerant angiosperm and conifer species. While being relatively intact compared to other forest biomes in Japan, subalpine coniferous forests are under significant threat from deer browsing, global warming and small population size effects. However, there is a severe lack of genetic resources available for this biome's major constituent plant species. This study aimed to develop chloroplast genome-based genetic resources for 12 widespread subalpine tree and shrub species (7 angiosperms and 5 conifers) via genome skimming of whole-genomic DNA using short reads (100-150 bp in length). For 10 species, whole chloroplast genomes were assembl

SUBMITTER: Worth JRP 

PROVIDER: S-EPMC11255381 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature