Integrative profiling of untreated primary membranous nephropathy at the single-cell transcriptome level.

ABSTRACT:

Background

Primary membranous nephropathy (PMN) is an autoimmune kidney disease. Despite the identification of certain autoantigens, the etiology and pathophysiology of PMN are still largely unknown.

Methods

Five patients with biopsy-proven PMN were enrolled in this study. Their blood, kidney and urine samples were collected respectively to profile cellular, molecular and immunological alterations by using single-cell RNA sequencing (scRNA-seq). Experimental verifications were also implemented in kidney tissue.

Results

In the peripheral blood mononuclear cell (PBMC) samples, portions of B cells and plasma cells were increased in PMN patients. Cell-cell communication analysis suggests that APRIL (a proliferation-inducing ligand from B cells) might be a potential

SUBMITTER: Gu Q 

PROVIDER: S-EPMC11255483 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature