Oligomerization of protein arginine methyltransferase 1 and its effect on methyltransferase activity and substrate specificity.

ABSTRACT: Proper protein arginine methylation by protein arginine methyltransferase 1 (PRMT1) is critical for maintaining cellular health, while dysregulation is often associated with disease. How the activity of PRMT1 is regulated is therefore paramount, but is not clearly understood. Several studies have observed higher order oligomeric species of PRMT1, but it is unclear if these exist at physiological concentrations and there is confusion in the literature about how oligomerization affects activity. We therefore sought to determine which oligomeric species of PRMT1 are physiologically relevant, and quantitatively correlate activity with specific oligomer forms. Through quantitative western blotting, we determined that concentrations of PRMT1 available in a variety of human cell lines are in the

SUBMITTER: Rossi V 

PROVIDER: S-EPMC11255602 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature