Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer.

ABSTRACT: Patient-derived organoid (PDO) models of cancer are a multifunctional research system that better recapitulates human disease as compared to cancer cell lines. PDO models can be generated by culturing patient tumor cells in extracellular basement membrane extracts (BME) and plating them as three-dimensional domes. However, commercially available reagents that have been optimized for phenotypic assays in monolayer cultures often are not compatible with BME. Herein, we describe a method to plate PDO models and assess drug effects using an automated live-cell imaging system. In addition, we apply fluorescent dyes that are compatible with kinetic measurements to quantify cell health and apoptosis simultaneously. Image capture can be customized to occur at regular time intervals over several da

SUBMITTER: Colling KE 

PROVIDER: S-EPMC11256050 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature