Metformin induces ZFP36 by mTORC1 inhibition in cervical cancer-derived cell lines.

ABSTRACT:

Background

Metformin, a widely prescribed antidiabetic drug, has shown several promising effects for cancer treatment. These effects have been shown to be mediated by dual modulation of the AMPK-mTORC1 axis, where AMPK acts upstream of mTORC1 to decrease its activity. Nevertheless, alternative pathways have been recently discovered suggesting that metformin can act through of different targets regulation.

Methods

We performed a transcriptome screening analysis using HeLa xenograft tumors generated in NOD-SCID mice treated with or without metformin to examine genes regulated by metformin. Western Blot analysis, Immunohistochemical staining, and RT-qPCR were used to confirm alterations in gene expression. The TNMplot and GEPIA2 platform were used for in silico analysis of gene

SUBMITTER: De la Cruz-Lopez KG 

PROVIDER: S-EPMC11256429 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature