MiR-29a-3p orchestrates key signaling pathways for enhanced migration of human mesenchymal stem cells.

ABSTRACT:

Background

The homing of human mesenchymal stem cells (hMSCs) is crucial for their therapeutic efficacy and is characterized by the orchestrated regulation of multiple signaling modules. However, the principal upstream regulators that synchronize these signaling pathways and their mechanisms during cellular migration remain largely unexplored.

Methods

miR-29a-3p was exogenously expressed in either wild-type or DiGeorge syndrome critical region 8 (DGCR8) knockdown hMSCs. Multiple pathway components were analyzed using Western blotting, immunohistochemistry, and real-time quantitative PCR. hMSC migration was assessed both in vitro and in vivo through wound healing, Transwell, contraction, and in vivo migration assays. Extensive bioinformatic analyses using gene set enrichment

SUBMITTER: Kang D 

PROVIDER: S-EPMC11256664 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature