Using a quantum dot bead-based lateral flow immunoassay to broadly detect the adulteration of PDE-5 inhibitors in functional foods.

ABSTRACT: In this study, a designed hapten possessing the classic structure of PDE-5 inhibitors was synthesized. A monoclonal antibody (mAb) with broad recognition for six PDE-5 inhibitors was further produced. For the determination of lodenafil, methisosildenafil, mirodenafil, udenafil and tadalafil, the limit of detection (LOD) and IC₅₀ ranged from 1.01 to 26.91 ng mL^-1 and 12.75 to 278 ng mL^-1, respectively. Thereafter, a quantum dot bead-based lateral flow immunoassay (QB-LFIA) was developed, which improved the LOD and IC₅₀ to 0.32-6.52 ng mL^-1 and 7.45-133.8 ng mL^-1, respectively. Method validation was conducted using honey and capsule samples spiked with PDE-5 inhibitors, and the recoveries of the intra- and inter-assays ranged

SUBMITTER: Song M 

PROVIDER: S-EPMC11257028 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature